Abstract:Reverse micelles combined enzyme hydrolysis was adopted to extract wheat germ protein.Reverse micelles system was formed by sulphosuccinic acid bis(2-ethylhexyl) ester sodium salt(AOT),isooctane and KCI buffer solution.Effects of extraction time,the PH of buffer solution and enzyme dosage on forward extraction condition,and the stationary point got from response surface model was the saddle point.The ridge analysis was developed to further study the results,and the maximum forward extraction efficiency of 76.99% was obtained at the condition of temperature 40¡æ,extraction time 1h,pH9.22 and enzyme dosage 0.153 AU/g.