Abstract:Reaction conditions for compound lipase catalyzed hydrolysis of soybean oil in a solvent-free system were investigated.Five different source  lipases of C.lipolytica(CLL),Sigma L-3126,PPL,Novo435 and Amano-G were screened by investigating the complex effect.Combining CLL and Amano-G could give a better hydrolysis rate.The optimum reaction conditions were obtained a follows:the dosage of CLL and Amano-G was 1% and 0.1%,respectively;Amano-G was added after CLL acted for 1h;reaction temperature was 45¡æ;water-phase was de-ionized water,and water/oil ratio was 1:1.Under above conditions,the hydrolysis rate increased from 45.7% in conventional shaking bath to 94.2% after reaction for 24h.